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Issue 3, 2012
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Glycodendrimer coated gold nanoparticles for proteins detection based on surface energy transfer process

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Abstract

We report here a novel and simple approach on which nanometer-size, water soluble glycodendrimer (mannose-functionalized poly(amidoamine) PAMAM generation G0 cystamine core dendrimer) coated gold nanoparticles (Au–man) allows the detection of proteincarbohydrate interactions based on surface energy transfer process (SET). This sensitive SET sensing approach enables quantitative analysis of the binding constant of a mannose-binding protein, lectin Concanavalin A (Con A) labelled with a fluorophore to a sensing Au–man in aqueous samples. Competitive binding and inhibition assays were done to investigate the SET efficiency. The binding constant of Con A to Au–man interactions of (5.6 ± 0.1) × 106 M−1 is 100-times higher than the binding constant values obtained in the interactions with the glycodendrimer G2 alone. The simple modification of the gold nanoparticles with glycodendrimers provides a convenient method to obtain biocompatible and selective carbohydrate-based SET probes for protein biorecognition.

Graphical abstract: Glycodendrimer coated gold nanoparticles for proteins detection based on surface energy transfer process

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Publication details

The article was received on 17 Oct 2011, accepted on 17 Oct 2011 and first published on 02 Dec 2011


Article type: Paper
DOI: 10.1039/C1RA00904D
RSC Adv., 2012,2, 985-991

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    Glycodendrimer coated gold nanoparticles for proteins detection based on surface energy transfer process

    N. Bogdan, R. Roy and M. Morin, RSC Adv., 2012, 2, 985
    DOI: 10.1039/C1RA00904D

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