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Issue 18, 2012
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Controlled immobilisation of active enzymes on the cowpea mosaic virus capsid

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Abstract

Immobilisation of horseradish peroxidase (HRP) and glucose oxidase (GOX) via covalent attachment of modified enzyme carbohydrate to the exterior of the cowpea mosaic virus (CPMV) capsid gave high retention of enzymatic activity. The number of enzymes bound per virus was determined to be about eleven for HRP and 2–3 for GOX. This illustrates that relatively large biomacromolecules can be readily coupled to the virus surface using simple conjugation strategies. Virus-biomacromolecule hybrids have great potential for uses in catalysis, diagnostic assays or biosensors.

Graphical abstract: Controlled immobilisation of active enzymes on the cowpea mosaic virus capsid

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Publication details

The article was received on 13 Jun 2012, accepted on 23 Jul 2012 and first published on 26 Jul 2012


Article type: Paper
DOI: 10.1039/C2NR31485A
Nanoscale, 2012,4, 5640-5645
  • Open access: Creative Commons BY license
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    Controlled immobilisation of active enzymes on the cowpea mosaic virus capsid

    A. A. A. Aljabali, J. E. Barclay, N. F. Steinmetz, G. P. Lomonossoff and D. J. Evans, Nanoscale, 2012, 4, 5640
    DOI: 10.1039/C2NR31485A

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