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Issue 10, 2012
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Insights from the sea: Structural biology of marine polyketide synthases

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Covering: up to the end of 2011

The world's oceans are a rich source of natural products with extremely interesting chemistry. Biosynthetic pathways have been worked out for a few, and the story is being enriched with crystal structures of interesting pathway enzymes. By far, the greatest number of structural insights from marine biosynthetic pathways has originated with studies of curacin A, a poster child for interesting marine chemistry with its cyclopropane and thiazoline rings, internal cis double bond, and terminal alkene. Using the curacin A pathway as a model, structural details are now available for a novel loading enzyme with remarkable dual decarboxylase and acetyltransferase activities, an Fe2+/α-ketoglutarate-dependent halogenase that dictates substrate binding order through conformational changes, a decarboxylase that establishes regiochemistry for cyclopropane formation, and a thioesterase with specificity for β-sulfated substrates that lead to terminal alkene offloading. The four curacin A pathway dehydratases reveal an intrinsic flexibility that may accommodate bulky or stiff polyketide intermediates. In the salinosporamide A pathway, active site volume determines the halide specificity of a halogenase that catalyzes for the synthesis of a halogenated building block. Structures of a number of putative polyketide cyclases may help in understanding reaction mechanisms and substrate specificities although their substrates are presently unknown.

Graphical abstract: Insights from the sea: Structural biology of marine polyketide synthases

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Publication details

The article was received on 03 Feb 2012 and first published on 13 Apr 2012

Article type: Review Article
DOI: 10.1039/C2NP20016C
Nat. Prod. Rep., 2012,29, 1038-1049

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    Insights from the sea: Structural biology of marine polyketide synthases

    D. L. Akey, J. J. Gehret, D. Khare and J. L. Smith, Nat. Prod. Rep., 2012, 29, 1038
    DOI: 10.1039/C2NP20016C

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