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Issue 22, 2012
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Fragmentation of DNA in a sub-microliter microfluidic sonication device

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Abstract

Fragmentation of DNA is an essential step for many biological applications including the preparation of next-generation sequencing (NGS) libraries. As sequencing technologies push the limits towards single cell and single molecule resolution, it is of great interest to reduce the scale of this upstream fragmentation step. Here we describe a miniaturized DNA shearing device capable of processing sub-microliter samples based on acoustic shearing within a microfluidic chip. A strong acoustic field was generated by a Langevin-type piezo transducer and coupled into the microfluidic channel via the flexural lamb wave mode. Purified genomic DNA, as well as covalently cross-linked chromatin were sheared into various fragment sizes ranging from ∼180 bp to 4 kb. With the use of standard PDMS soft lithography, our approach should facilitate the integration of additional microfluidic modules and ultimately allow miniaturized NGS workflows.

Graphical abstract: Fragmentation of DNA in a sub-microliter microfluidic sonication device

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Article information


Submitted
23 May 2012
Accepted
04 Sep 2012
First published
26 Sep 2012

Lab Chip, 2012,12, 4677-4682
Article type
Technical Innovation

Fragmentation of DNA in a sub-microliter microfluidic sonication device

Q. Tseng, A. M. Lomonosov, E. E. M. Furlong and C. A. Merten, Lab Chip, 2012, 12, 4677
DOI: 10.1039/C2LC40595D

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