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Issue 14, 2012
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High-throughput biophysical measurement of human red blood cells

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Abstract

This paper reports a microfluidic system for biophysical characterization of red blood cells (RBCs) at a speed of 100–150 cells s−1. Electrical impedance measurement is made when single RBCs flow through a constriction channel that is marginally smaller than RBCs' diameters. The multiple parameters quantified as mechanical and electrical signatures of each RBC include transit time, impedance amplitude ratio, and impedance phase increase. Histograms, compiled from 84,073 adult RBCs (from 5 adult blood samples) and 82,253 neonatal RBCs (from 5 newborn blood samples), reveal different biophysical properties across samples and between the adult and neonatal RBC populations. In comparison with previously reported microfluidic devices for single RBC biophysical measurement, this system has a higher throughput, higher signal to noise ratio, and the capability of performing multi-parameter measurements.

Graphical abstract: High-throughput biophysical measurement of human red blood cells

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Article information


Submitted
06 Dec 2011
Accepted
26 Mar 2012
First published
14 May 2012

Lab Chip, 2012,12, 2560-2567
Article type
Paper

High-throughput biophysical measurement of human red blood cells

Y. Zheng, E. Shojaei-Baghini, A. Azad, C. Wang and Y. Sun, Lab Chip, 2012, 12, 2560
DOI: 10.1039/C2LC21210B

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