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Issue 7, 2012
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Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform

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Abstract

We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynamic conditions. The impact parameters governing the occupancy distribution and capture efficiency of the arrayed traps are investigated. The unique, nearly 100% capture efficiency paired with the capability to establish sharply peaked, single occupancy distributions enables a novel, digital readout mode for color-multiplexed, particle-based assays with low-complexity instrumentation. The presented technology marks an essential step towards a versatile platform for the integration of bead- and cell-based biological assays.

Graphical abstract: Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform

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Article information


Submitted
28 Nov 2011
Accepted
03 Feb 2012
First published
15 Feb 2012

Lab Chip, 2012,12, 1289-1295
Article type
Paper

Array-based capture, distribution, counting and multiplexed assaying of beads on a centrifugal microfluidic platform

R. Burger, P. Reith, G. Kijanka, V. Akujobi, P. Abgrall and J. Ducrée, Lab Chip, 2012, 12, 1289
DOI: 10.1039/C2LC21170J

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