Jump to main content
Jump to site search

Issue 8, 2012
Previous Article Next Article

Electrochemistry-enabled fabrication of orthogonal nanotopography and surface chemistry gradients for high-throughput screening

Author affiliations

Abstract

Gradient surfaces are emerging tools for investigating mammalian cell-surface interactions in high throughput. We demonstrate the electrochemical fabrication of an orthogonal gradient platform combining a porous silicon (pSi) pore size gradient with an orthogonal gradient of peptide ligand density. pSi gradients were fabricated via the anodic etching of a silicon wafer with pore sizes ranging from hundreds to tens of nanometers. A chemical gradient of ethyl-6-bromohexanoate was generated orthogonally to the pSi gradient via electrochemical attachment. Subsequent hydrolysis and activation of the chemical gradient allowed for the generation of a cyclic RGD gradient. Whilst mesenchymal stem cells (MSC) were shown to respond to both the topographical and chemical cues arising from the orthogonal gradient, the MSC's responded more strongly to changes in RGD density than to changes in pore size during short-term culture.

Graphical abstract: Electrochemistry-enabled fabrication of orthogonal nanotopography and surface chemistry gradients for high-throughput screening

Back to tab navigation

Supplementary files

Article information


Submitted
09 Aug 2011
Accepted
17 Feb 2012
First published
07 Mar 2012

Lab Chip, 2012,12, 1480-1486
Article type
Paper

Electrochemistry-enabled fabrication of orthogonal nanotopography and surface chemistry gradients for high-throughput screening

L. R. Clements, P. Wang, W. Tsai, H. Thissen and N. H. Voelcker, Lab Chip, 2012, 12, 1480
DOI: 10.1039/C2LC20732J

Social activity

Search articles by author

Spotlight

Advertisements