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Issue 23, 2012
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Micropatterning of neural stem cells and Purkinje neurons using a polydimethylsiloxane (PDMS) stencil

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Abstract

A new fabrication method of a polydimethylsiloxane (PDMS) stencil embedded microwell plate is proposed and applied to a localized culture of Purkinje neurons (PNs) and neural stem cells (NSCs). A microwell plate combines a PDMS stencil and well plate. The PDMS stencil was fabricated by spin casting from an SU-8 master mold. Gas blowing using nitrogen was adopted to perforate the stencil membrane. An acrylic well plate compartment mold was fabricated using computer numerical control (CNC) machining. By PDMS casting using a stencil placed on an acrylic mold, microwell plates were fabricated without punching or the use of a plasma bonding process. By using the stencil as a physical mask for the cell culture, PNs and NSCs were successfully cultured into micropatterns. The microwell plate could be applied to the localizing and culturing of a cell. The micropatterned NSCs were differentiated into neurons, astrocytes, and oligodendrocytes. The results showed that cells could be cultured and differentiated into micropatterns in a precisely controlled manner in any shape and in specific sizes for bioscience study and bioengineering applications.

Graphical abstract: Micropatterning of neural stem cells and Purkinje neurons using a polydimethylsiloxane (PDMS) stencil

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Article information


Submitted
06 Jul 2012
Accepted
05 Sep 2012
First published
06 Sep 2012

Lab Chip, 2012,12, 5045-5050
Article type
Paper

Micropatterning of neural stem cells and Purkinje neurons using a polydimethylsiloxane (PDMS) stencil

J. H. Choi, H. Lee, H. K. Jin, J. Bae and G. M. Kim, Lab Chip, 2012, 12, 5045
DOI: 10.1039/C2LC40764G

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