Jump to main content
Jump to site search

Issue 20, 2012
Previous Article Next Article

Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times

Author affiliations

Abstract

Although polymerization-based amplification (PBA) has demonstrated promise as an inexpensive technique for use in molecular diagnostics, oxygen inhibition of radical photopolymerization has hindered its implementation in point-of-care devices. The addition of 0.3–0.7 μM eosin to an aqueous acrylate monomer solution containing a tertiary amine allows an interfacial polymerization reaction to proceed in air only near regions of a test surface where additional eosin initiators coupled to proteins have been localized as a function of molecular recognition events. The dose of light required for the reaction is inversely related to eosin concentration. This system achieves sensitivities comparable to those reported for inert gas-purged systems and requires significantly shorter reaction times. We provide several comparisons of this system with other implementations of polymerization-based amplification.

Graphical abstract: Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times

Back to tab navigation

Supplementary files

Article information


Submitted
21 May 2012
Accepted
13 Aug 2012
First published
14 Aug 2012

Lab Chip, 2012,12, 4055-4058
Article type
Technical Innovation

Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times

K. Kaastrup and H. D. Sikes, Lab Chip, 2012, 12, 4055 DOI: 10.1039/C2LC40584A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements