Jump to main content
Jump to site search

Issue 20, 2012
Previous Article Next Article

Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times

Author affiliations

Abstract

Although polymerization-based amplification (PBA) has demonstrated promise as an inexpensive technique for use in molecular diagnostics, oxygen inhibition of radical photopolymerization has hindered its implementation in point-of-care devices. The addition of 0.3–0.7 μM eosin to an aqueous acrylate monomer solution containing a tertiary amine allows an interfacial polymerization reaction to proceed in air only near regions of a test surface where additional eosin initiators coupled to proteins have been localized as a function of molecular recognition events. The dose of light required for the reaction is inversely related to eosin concentration. This system achieves sensitivities comparable to those reported for inert gas-purged systems and requires significantly shorter reaction times. We provide several comparisons of this system with other implementations of polymerization-based amplification.

Graphical abstract: Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times

Back to tab navigation

Supplementary files

Article information


Submitted
21 May 2012
Accepted
13 Aug 2012
First published
14 Aug 2012

Lab Chip, 2012,12, 4055-4058
Article type
Technical Innovation

Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times

K. Kaastrup and H. D. Sikes, Lab Chip, 2012, 12, 4055
DOI: 10.1039/C2LC40584A

Social activity

Search articles by author

Spotlight

Advertisements