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Issue 22, 2012
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Prediction and control of number of cells in microdroplets by stochastic modeling

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Abstract

Manipulation and encapsulation of cells in microdroplets has found many applications in various fields such as clinical diagnostics, pharmaceutical research, and regenerative medicine. The control over the number of cells in individual droplets is important especially for microfluidic and bioprinting applications. There is a growing need for modeling approaches that enable control over a number of cells within individual droplets. In this study, we developed statistical models based on negative binomial regression to determine the dependence of number of cells per droplet on three main factors: cell concentration in the ejection fluid, droplet size, and cell size. These models were based on experimental data obtained by using a microdroplet generator, where the presented statistical models estimated the number of cells encapsulated in droplets. We also propose a stochastic model for the total volume of cells per droplet. The statistical and stochastic models introduced in this study are adaptable to various cell types and cell encapsulation technologies such as microfluidic and acoustic methods that require reliable control over number of cells per droplet provided that settings and interaction of the variables is similar.

Graphical abstract: Prediction and control of number of cells in microdroplets by stochastic modeling

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Supplementary files

Article information


Submitted
12 Oct 2011
Accepted
30 May 2012
First published
30 May 2012

Lab Chip, 2012,12, 4884-4893
Article type
Paper

Prediction and control of number of cells in microdroplets by stochastic modeling

E. Ceyhan, F. Xu, U. A. Gurkan, A. E. Emre, E. S. Turali, R. El Assal, A. Acikgenc, C. M. Wu and U. Demirci, Lab Chip, 2012, 12, 4884
DOI: 10.1039/C2LC40523G

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