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Issue 3, 2012
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Polymer-monovalent salt-induced DNA compaction studied via single-molecule microfluidic trapping

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Abstract

Polymer-monovalent salt-induced single-molecule DNA compaction/condensation in a microfluidic stagnation point flow was studied by analyzing both DNA compaction images and time trajectories. For the whole DNA compaction process we observed three successive steps: Step I, a relaxation process of the stretched DNA that occurs slowly along the whole DNA chain, Step II, nucleus formation and growth, and Step III, corresponding to a rapid compaction of the chain. A memory effect was observed between Steps I and III, and a new (intruder-induced) nucleation mode was observed for the first time. This study extends the use of the microfluidic stagnation point flow, which we have previously used for sequence detection and to measure enzyme kinetics site-specifically.

Graphical abstract: Polymer-monovalent salt-induced DNA compaction studied via single-molecule microfluidic trapping

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Publication details

The article was received on 14 Sep 2011, accepted on 28 Nov 2011 and first published on 16 Dec 2011


Article type: Paper
DOI: 10.1039/C2LC20880F
Lab Chip, 2012,12, 647-651

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    Polymer-monovalent salt-induced DNA compaction studied via single-molecule microfluidic trapping

    W. Xu and S. J. Muller, Lab Chip, 2012, 12, 647
    DOI: 10.1039/C2LC20880F

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