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Issue 1, 2012
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Flexible planar microfluidic chip employing a light emitting diode and a PIN-photodiode for portable flow cytometers

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Abstract

Detection of fluorescence particles is a key method of flow cytometry. We evaluate the performance of a design for a microfluidic fluorescence particle detection device. Due to the planar design with low layer thicknesses, we avoid optical components such as lenses or dichroic mirrors and substitute them with a shadow mask and colored film filters. A commercially available LED is used as the light source and a PIN-photodiode as detector. This design approach reduces component cost and power consumption and enables supplying the device with power from a standard USB port. From evaluation of this design, we obtain a maximum particle detection frequency of up to 600 particles per second at a sensitivity of better than 4.7 × 105 MESF (molecules of equivalent soluble fluorochrome) measured with particles for FITC sensitivity calibration. Lowering the flow rate increases the instrument sensitivity by an order of magnitude enabling the detection of particles with 4.5 × 104 MESF.

Graphical abstract: Flexible planar microfluidic chip employing a light emitting diode and a PIN-photodiode for portable flow cytometers

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Article information


Submitted
22 Jul 2011
Accepted
11 Oct 2011
First published
15 Nov 2011

Lab Chip, 2012,12, 197-203
Article type
Paper

Flexible planar microfluidic chip employing a light emitting diode and a PIN-photodiode for portable flow cytometers

S. W. Kettlitz, S. Valouch, W. Sittel and U. Lemmer, Lab Chip, 2012, 12, 197
DOI: 10.1039/C1LC20672A

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