Jump to main content
Jump to site search

Issue 1, 2012
Previous Article Next Article

Flexible planar microfluidic chip employing a light emitting diode and a PIN-photodiode for portable flow cytometers

Author affiliations

Abstract

Detection of fluorescence particles is a key method of flow cytometry. We evaluate the performance of a design for a microfluidic fluorescence particle detection device. Due to the planar design with low layer thicknesses, we avoid optical components such as lenses or dichroic mirrors and substitute them with a shadow mask and colored film filters. A commercially available LED is used as the light source and a PIN-photodiode as detector. This design approach reduces component cost and power consumption and enables supplying the device with power from a standard USB port. From evaluation of this design, we obtain a maximum particle detection frequency of up to 600 particles per second at a sensitivity of better than 4.7 × 105 MESF (molecules of equivalent soluble fluorochrome) measured with particles for FITC sensitivity calibration. Lowering the flow rate increases the instrument sensitivity by an order of magnitude enabling the detection of particles with 4.5 × 104 MESF.

Graphical abstract: Flexible planar microfluidic chip employing a light emitting diode and a PIN-photodiode for portable flow cytometers

Back to tab navigation

Supplementary files

Publication details

The article was received on 22 Jul 2011, accepted on 11 Oct 2011 and first published on 15 Nov 2011


Article type: Paper
DOI: 10.1039/C1LC20672A
Lab Chip, 2012,12, 197-203

  •   Request permissions

    Flexible planar microfluidic chip employing a light emitting diode and a PIN-photodiode for portable flow cytometers

    S. W. Kettlitz, S. Valouch, W. Sittel and U. Lemmer, Lab Chip, 2012, 12, 197
    DOI: 10.1039/C1LC20672A

Search articles by author

Spotlight

Advertisements