Jump to main content
Jump to site search

Issue 9, 2012
Previous Article Next Article

Caffeine dose-dependently induces thermogenesis but restores ATP in HepG2 cells in culture

Author affiliations

Abstract

Caffeine has been hypothesised as a thermogenic agent that might help to maintain a healthy body weight. Since very little is known about its actions on cellular energy metabolism, we investigated the effect of caffeine on mitochondrial oxidative phosphorylation, cellular energy supply and thermogenesis in HepG2 cells, and studied its action on fatty acid uptake and lipid accumulation in 3T3-L1 adipocytes at concentrations ranging from 30–1500 μM. In HepG2 cells, caffeine induced a depolarisation of the inner mitochondrial membrane, a feature of mitochondrial thermogenesis, both directly and after 24 h incubation. Increased concentrations of uncoupling protein-2 (UCP-2) also indicated a thermogenic activity of caffeine. Energy generating pathways, such as mitochondrial respiration, fatty acid oxidation and anaerobic lactate production, were attenuated by caffeine treatment. Nevertheless, HepG2 cells demonstrated a higher energy charge potential after exposure to caffeine that might result from energy restoration through attenuation of energy consuming pathways, as typically found in hibernating animals. In 3T3-L1 cells, in contrast, caffeine increased fatty acid uptake, but did not affect lipid accumulation. We provide evidence that caffeine stimulates thermogenesis but concomitantly causes energy restoration that may compensate enhanced energy expenditure.

Graphical abstract: Caffeine dose-dependently induces thermogenesis but restores ATP in HepG2 cells in culture

Back to tab navigation

Publication details

The article was received on 04 Mar 2012, accepted on 05 May 2012 and first published on 08 May 2012


Article type: Paper
DOI: 10.1039/C2FO30053B
Food Funct., 2012,3, 955-964

  •   Request permissions

    Caffeine dose-dependently induces thermogenesis but restores ATP in HepG2 cells in culture

    A. Riedel, M. Pignitter, C. M. Hochkogler, B. Rohm, J. Walker, G. Bytof, I. Lantz and V. Somoza, Food Funct., 2012, 3, 955
    DOI: 10.1039/C2FO30053B

Search articles by author

Spotlight

Advertisements