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Issue 55, 2012
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Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction

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Abstract

A method for enzymatic production of short (10–20 nt) cytosine-modified oligonucleotides was developed by nicking enzyme amplification reaction using Vent(exo-) polymerase, Nt.BstNBI nicking endonuclease and 5-substituted dCTP derivatives. The methodology including isolation was scaled up to nanomolar amounts and was proved to be suitable for production of diverse base-modified short single-stranded oligonucleotides (inaccessible by other enzymatic methods) that are of potential interest as labelled primers or functionalized aptamers.

Graphical abstract: Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction

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Article information


Submitted
24 Apr 2012
Accepted
15 May 2012
First published
16 May 2012

Chem. Commun., 2012,48, 6921-6923
Article type
Communication

Preparation of short cytosine-modified oligonucleotides by nicking enzyme amplification reaction

P. Ménová and M. Hocek, Chem. Commun., 2012, 48, 6921
DOI: 10.1039/C2CC32930A

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