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Issue 11, 2012
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A surface-based mass spectrometry method for screening glycosidase specificity in environmental samples

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Abstract

A new surface-based MALDI-Tof-MS glycosyl hydrolase assay has been developed in which lipid-tagged oligosaccharides, representing defined fragments of major plant cell wall polysaccharides, are immobilized via hydrophobic interactions on an alkylthiol functionalised gold sample plate and employed in the functional screening of several purified enzymes, environmental samples and saliva.

Graphical abstract: A surface-based mass spectrometry method for screening glycosidase specificity in environmental samples

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Publication details

The article was received on 20 Oct 2011, accepted on 02 Dec 2011 and first published on 06 Dec 2011


Article type: Communication
DOI: 10.1039/C2CC16537F
Citation: Chem. Commun., 2012,48, 1701-1703
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    A surface-based mass spectrometry method for screening glycosidase specificity in environmental samples

    A. Beloqui, A. Sanchez-Ruiz, M. Martin-Lomas and N. Reichardt, Chem. Commun., 2012, 48, 1701
    DOI: 10.1039/C2CC16537F

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