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Issue 23, 2012
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Indirect competitive immunoassay for the detection of fungicideThiabendazole in whole orange samples by Surface Plasmon Resonance

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Abstract

A highly sensitive and specific SPR-based competitive immunoassay for the detection of Thiabendazole (TBZ) has been developed. An indirect format where a TBZ–protein conjugate is immobilized onto gold surfaces has been selected. Under the optimal conditions, a LOD of 0.67 nM (0.13 μg L−1) and an IC50 of 3.2 nM (0.64 μg L−1) have been achieved which are comparable to the values obtained by conventional ELISA. Analysis of real samples has been attempted by first evaluating the influence of complex matrix samples coming from whole oranges and secondly measuring samples containing TBZ previously evaluated by chromatographic methods. A methanolic extraction procedure followed by a simple dilution in assay buffer has proven to be sufficient to measure orange samples using the developed immunoassay with an excellent recovery percentage. The sensitivity and the feasibility of measuring whole orange samples demonstrate the effectiveness and robustness of the SPR biosensor, which can be useful for the determination of TBZ in food at concentrations below the Maximum Residue Levels (MRLs) established by the European legislation.

Graphical abstract: Indirect competitive immunoassay for the detection of fungicide Thiabendazole in whole orange samples by Surface Plasmon Resonance

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Publication details

The article was received on 09 Aug 2012, accepted on 22 Sep 2012 and first published on 25 Sep 2012


Article type: Paper
DOI: 10.1039/C2AN36094B
Analyst, 2012,137, 5659-5665

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    Indirect competitive immunoassay for the detection of fungicide Thiabendazole in whole orange samples by Surface Plasmon Resonance

    M.-Carmen Estevez, J. Belenguer, S. Gomez-Montes, J. Miralles, A. M. Escuela, A. Montoya and L. M. Lechuga, Analyst, 2012, 137, 5659
    DOI: 10.1039/C2AN36094B

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