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Issue 16, 2012
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Color-encoded microcarriers based on nano-silicon dioxide film for multiplexed immunoassays

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Abstract

Multiplexed analysis allows researchers to obtain high-density information with minimal assay time, sample volume and cost. Currently, microcarrier or particle-based approaches for multiplexed analysis involve complicated or expensive encoding and decoding processes. In this paper, a novel optical encoding technique based on nano-silicon dioxide film is presented. Microcarriers composed of thermally grown silicon dioxide (SiO2) film and monocrystalline silicon (Si) substrate were fabricated. The nano-silicon dioxide film exhibited unique surface color by low-coherence interference. Hence the colors can be used for encoding at least 100 microcarriers loaded with films of different thickness. We demonstrated that color-encoded microcarriers loaded with antigens could be used for multiplexed immunoassays to detect goat anti-human IgG, goat anti-mouse IgG and goat anti-rabbit IgG, with fluorescent detection as the interrogating approach. This microcarrier-based method also exhibited improved analytical performance compared with a microarray technique. This approach will provide new opportunities for multiplexed target assay development.

Graphical abstract: Color-encoded microcarriers based on nano-silicon dioxide film for multiplexed immunoassays

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Publication details

The article was received on 27 Mar 2012, accepted on 24 May 2012 and first published on 24 May 2012


Article type: Paper
DOI: 10.1039/C2AN35410A
Analyst, 2012,137, 3760-3766

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    Color-encoded microcarriers based on nano-silicon dioxide film for multiplexed immunoassays

    Q. Li, K. Zhang, T. Wang, X. Zhou, J. Wang, C. Wang, H. Lin, X. Li, Y. Lu and G. Huang, Analyst, 2012, 137, 3760
    DOI: 10.1039/C2AN35410A

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