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Issue 24, 2012
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A bacteriophage endolysin-based electrochemical impedance biosensor for the rapid detection of Listeria cells

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Abstract

The objective of this study was to develop a biosensor using the cell wall binding domain (CBD) of bacteriophage-encoded peptidoglycan hydrolases (endolysin) immobilized on a gold screen printed electrode (SPE) and subsequent electrochemical impedance spectroscopy (EIS) for a rapid and specific detection of Listeria cells. The endolysin was amine-coupled to SPEs using EDC/NHS chemistry. The CBD-based electrode was used to capture and detect the Listeria innocua serovar 6b from pure culture and 2% artificially contaminated milk. In our study, the endolysin functionalized SPEs have been characterized using X-ray photoelectron spectroscopy (XPS). The integration of endolysin-based recognition for specific bacteria and EIS can be used for direct and rapid detection of Listeria cells with high specificity against non-Listeria cells with a limit of detection of 1.1 × 104 and 105 CFU mL−1 in pure culture and 2% milk, respectively.

Graphical abstract: A bacteriophage endolysin-based electrochemical impedance biosensor for the rapid detection of Listeria cells

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Publication details

The article was received on 20 Jul 2012, accepted on 19 Sep 2012 and first published on 20 Sep 2012


Article type: Paper
DOI: 10.1039/C2AN35988J
Analyst, 2012,137, 5749-5756

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    A bacteriophage endolysin-based electrochemical impedance biosensor for the rapid detection of Listeria cells

    M. Tolba, M. U. Ahmed, C. Tlili, F. Eichenseher, M. J. Loessner and M. Zourob, Analyst, 2012, 137, 5749
    DOI: 10.1039/C2AN35988J

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