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Issue 24, 2012
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Three-dimensional Raman spectroscopic imaging of protein crystals deposited on a nanodroplet

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Abstract

Confocal Raman spectroscopic imaging has been used to find the location of protein crystals deposited in a nanodroplet. The depth of the protein crystal has been clearly identified by comparing the three-dimensional Raman spectroscopic images of the protein with those of water. Additionally, the low concentration region around a growing protein crystal in the nanodroplet was visualized using two-dimensional Raman spectroscopic imaging.

Graphical abstract: Three-dimensional Raman spectroscopic imaging of protein crystals deposited on a nanodroplet

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Article information


Submitted
11 Jul 2012
Accepted
02 Oct 2012
First published
02 Oct 2012

Analyst, 2012,137, 5730-5735
Article type
Paper

Three-dimensional Raman spectroscopic imaging of protein crystals deposited on a nanodroplet

S. Nitahara, M. Maeki, H. Yamaguchi, K. Yamashita, M. Miyazaki and H. Maeda, Analyst, 2012, 137, 5730
DOI: 10.1039/C2AN35942A

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