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Issue 22, 2012
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Electrokinetic focusing and separation of mammalian cells in conductive biological fluids

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Abstract

Active manipulation of cells, such as trapping, focusing, and isolation, is essential for various bioanalytical applications. Herein, we report a hybrid electrokinetic technique for manipulating mammalian cells in physiological fluids. This technique applies a combination of negative dielectrophoretic force and hydrodynamic drag force induced by electrohydrodynamics, which is effective in conductive biological fluids. With a three-electrode configuration, the stable equilibrium positions of cells can be adjusted for separation and focusing applications. Cancer cells and white blood cells can be positioned and isolated into specific locations in the microchannel under both static and dynamic flow conditions. To investigate the sensitivity of the hybrid electrokinetic process, AC voltage, frequency, and bias dependences of the cell velocity were studied systematically. The applicability of the hybrid electrokinetic technique for manipulating cells in physiological samples is demonstrated by continuous focusing of human breast adenocarcinoma spiked in urine, buffy coats, and processed blood samples with 98% capture efficiency.

Graphical abstract: Electrokinetic focusing and separation of mammalian cells in conductive biological fluids

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Supplementary files

Article information


Submitted
28 May 2012
Accepted
14 Aug 2012
First published
31 Aug 2012

Analyst, 2012,137, 5215-5221
Article type
Paper

Electrokinetic focusing and separation of mammalian cells in conductive biological fluids

J. Gao, R. Riahi, M. L. Y. Sin, S. Zhang and P. K. Wong, Analyst, 2012, 137, 5215
DOI: 10.1039/C2AN35707K

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