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Issue 18, 2012
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Label-free molecular analysis of live Neospora caninum tachyzoites in host cells by selective scanning Raman micro-spectroscopy

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Abstract

A selective scanning method was used to measure spatially resolved Raman spectra of live Neospora caninum tachyzoites colonizing human brain microvascular-endothelial cells. The technique allowed the detection of nucleic acids, lipids and proteins linked to the parasites and their cellular micro-environment at ∼10× shorter acquisition time compared to raster scanning.

Graphical abstract: Label-free molecular analysis of live Neospora caninum tachyzoites in host cells by selective scanning Raman micro-spectroscopy

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Supplementary files

Article information


Submitted
16 May 2012
Accepted
19 Jul 2012
First published
20 Jul 2012

Analyst, 2012,137, 4119-4122
Article type
Communication

Label-free molecular analysis of live Neospora caninum tachyzoites in host cells by selective scanning Raman micro-spectroscopy

K. Kong, C. J. Rowlands, H. Elsheikha and I. Notingher, Analyst, 2012, 137, 4119
DOI: 10.1039/C2AN35640F

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