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Issue 5, 2012
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Streptococcus suis II immunoassay based on thorny gold nanoparticles and surface enhanced Raman scattering

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Abstract

An immunoassay based on surface enhanced Raman scattering (SERS) spectroscopy was developed to detect muramidase released protein (MRP) antibody against Streptococcus suis II (SS2) utilizing thorny gold nanoparticles (tAuNPs) as SERS substrates. Initially, tAuNPs with multi-branches were prepared by the seed-mediated growth method in the absence of templates and surfactants, facilitating p-mercaptobenzoic acid (pMBA) conjugation covalently onto the tAuNPs through S–Au bonds. The obtained immuno-SERS tag affording strong Raman signals made it possible to establish an application of indirect detection of the MRP antibody against SS2 with a sandwich assay at a highly sensitive level. The Raman intensity at 1588 cm−1 was proportional to the logarithm of the concentration of MRP antibody in the range of 10 pg mL−1 to 0.1 μg mL−1. The detection sensitivity was significantly improved to 0.1 pg mL−1 by using the immuno-SERS tags. Furthermore, the proposed SERS approach was applied to detect MRP antibody in pig serum samples, and the results agreed well with those of ELISA, indicating great potential for clinical application in diagnostic immunoassays.

Graphical abstract: Streptococcus suis II immunoassay based on thorny gold nanoparticles and surface enhanced Raman scattering

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Publication details

The article was received on 22 Oct 2011, accepted on 27 Dec 2011 and first published on 26 Jan 2012


Article type: Paper
DOI: 10.1039/C2AN15997J
Analyst, 2012,137, 1259-1264

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    Streptococcus suis II immunoassay based on thorny gold nanoparticles and surface enhanced Raman scattering

    K. Chen, H. Han and Z. Luo, Analyst, 2012, 137, 1259
    DOI: 10.1039/C2AN15997J

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