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Issue 3, 2012
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Quantitative analysis of urea in human urine and serum by 1H nuclear magnetic resonance

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Abstract

A convenient and fast method for quantifying urea in biofluids is demonstrated using NMR analysis and the solvent water signal as a concentration reference. The urea concentration can be accurately determined with errors less than 3% between 1 mM and 50 mM, and less than 2% above 50 mM in urine and serum. The method is promising for various applications with advantages of simplicity, high accuracy, and fast non-destructive detection. With an ability to measure other metabolites simultaneously, this NMR method is also likely to find applications in metabolic profiling and system biology.

Graphical abstract: Quantitative analysis of urea in human urine and serum by 1H nuclear magnetic resonance

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Publication details

The article was received on 26 Aug 2011, accepted on 05 Dec 2011 and first published on 16 Dec 2011


Article type: Paper
DOI: 10.1039/C2AN15780B
Analyst, 2012,137, 595-600

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    Quantitative analysis of urea in human urine and serum by 1H nuclear magnetic resonance

    L. Liu, H. Mo, S. Wei and D. Raftery, Analyst, 2012, 137, 595
    DOI: 10.1039/C2AN15780B

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