Issue 2, 2011
From the journal:

Soft Matter

Nanocompartments with a pH release system based on an engineered OmpF channel protein†

Saskia Ihle,ad   Ozana Onaca,b   Per Rigler,b   Bernhard Hauer,c   Francisco Rodríguez-Ropero,d   Marco Fioronid  and  Ulrich Schwaneberg*d  

* Corresponding authors

a School of Engineering and Science, Jacobs University, Campus Ring 8, Bremen, Germany

b Department of Chemistry, University of Basel, Klingelbergstrasse 80, Basel, Switzerland

c Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, Stuttgart, Germany

d RWTH Aachen University, Worringer Weg 1, Aachen, Germany
E-mail: u.schwaneberg@biotec.rwth-aachen.de
Fax: +49 241 80 22387
Tel: +49 241 80 24170

Abstract

Synthosomes are a subclass of Polymersomes with a block copolymer membrane (PMOXA–PDMS–PMOXA) and a modified embedded transmembrane channel protein which acts as a selective gate. A synthosome based pH release system functioning increasing the pH from 5 to 7 was developed by introducing six histidine mutations to the OmpF constriction site (OmpF 6His). The pH-dependent compound release (acridine orange, positively charged between 5 ≤ pH ≤ 7) has been found to be tuned by the constriction site electrostatics and size by local structural modifications.

Graphical abstract: Nanocompartments with a pH release system based on an engineered OmpF channel protein

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Article information

DOI
https://doi.org/10.1039/C0SM00679C
Article type
Paper
Submitted
14 Jul 2010
Accepted
15 Oct 2010
First published
11 Nov 2010

Soft Matter, 2011,7, 532-539

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Nanocompartments with a pH release system based on an engineered OmpF channel protein

S. Ihle, O. Onaca, P. Rigler, B. Hauer, F. Rodríguez-Ropero, M. Fioroni and U. Schwaneberg, Soft Matter, 2011, 7, 532 DOI: 10.1039/C0SM00679C

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