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Issue 4, 2011
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Single molecule fluorescence spectroscopy: a tool for protein studies approaching cellular environmental conditions

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Abstract

Due to their high sensitivity and specificity fluorescence based single molecule techniques offer the possibility to study individual molecules (e.g., proteins or protein complexes) in situ in their cellular context. Recent progress in instrumentation and in sample preparation provides an increasingly better accessibility to more complex molecular assemblies. These assemblies mimic the natural cellular environmental conditions and at the same time allow sophisticated studies on proteins of interest. This review gives a brief introduction to single molecule fluorescence techniques and presents some selected applications on protein folding and on complex formation of membrane proteins.

Graphical abstract: Single molecule fluorescence spectroscopy: a tool for protein studies approaching cellular environmental conditions

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Article information


Submitted
16 Jun 2010
Accepted
16 Sep 2010
First published
09 Oct 2010

Soft Matter, 2011,7, 1254-1259
Article type
Emerging Area

Single molecule fluorescence spectroscopy: a tool for protein studies approaching cellular environmental conditions

J. Fitter, A. Katranidis, T. Rosenkranz, D. Atta, R. Schlesinger and G. Büldt, Soft Matter, 2011, 7, 1254
DOI: 10.1039/C0SM00538J

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