1H NMR metabolomics combined with gene expression analysis for the determination of major metabolic differences between subtypes of breast cell lines

Abstract

¹H NMR analysis was performed on metabolic extracts from a selection of six breast cell lines, including normal-immortalized, invasive ductal carcinomas and adenocarcinomas. Metabolites with significant concentration differences between normal and cancerous cells as well as ER+ and ER− (estrogen receptor) cells were determined and their relation to the differentially expressed genes was explored. Major differences have been shown for many amino acids and this was linked to expression level changes of related genes. Observed changes in choline concentration were connected to expression level changes of the SCL44A1 transporter gene.