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Issue 8, 2011
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Integrated in situ genetic analyzer for microbiology in extreme environments

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Abstract

In this study, a totally integrated in situ analyzer for microbial gene detection has been developed for oceanography applications. A PDMS–glass microfluidic device that is capable of cell lysis, DNA purification, PCR, and optical detection has been utilized as the core element of the in situ analyzer. Microbial genomic DNA is purified and concentrated on glass beads packed in the microfluidic device. PCR is performed in a flow-through manner, and the amplified products are fluorescently detected using optical fibers. The sensitivity of a completed analyzer with deep-sea operation capabilities has been evaluated in a laboratory setting, and the analyzer has been operated in real deep-sea environments. Field evaluations have shown that the amplification of the eubacterial universal 16S rRNA gene and the recovery of the PCR product to the surface are successfully achieved in deep-sea environments.

Graphical abstract: Integrated in situ genetic analyzer for microbiology in extreme environments

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Publication details

The article was received on 20 Jul 2011, accepted on 22 Aug 2011 and first published on 21 Oct 2011


Article type: Paper
DOI: 10.1039/C1RA00490E
RSC Adv., 2011,1, 1567-1573

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    Integrated in situ genetic analyzer for microbiology in extreme environments

    T. Fukuba, A. Miyaji, T. Okamoto, T. Yamamoto, S. Kaneda and T. Fujii, RSC Adv., 2011, 1, 1567
    DOI: 10.1039/C1RA00490E

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