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Abstract | Cited by

A novel strategy to systematically tune the pKa values of rhodamines is described. This strategy was applied to rationally develop compound 1e with a pKa of 6.5, the highest among rhodamine amide derivatives, and it could be employed to detect acidic pH variations in living cells with a turn-on signal.

Graphical abstract: A rational approach to tuning the pKa values of rhodamines for living cell fluorescence imaging

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