Issue 1, 2011
From the journal:

Organic & Biomolecular Chemistry

“Click-made” biaryl-linker improving efficiency in proteinlabelling for the membrane target protein of a bioactive compound†

Yoko Nakamura,a   Sho Inomata,a   Makoto Ebine,a   Yoshiyuki Manabe,a   Izumi Iwakurab  and  Minoru Ueda*a  

* Corresponding authors

a Department of Chemistry, Tohoku University, 6-3 Aramaki-aza Aoba, Aoba-ku, Sendai 980-8578, Japan
E-mail: ueda@m.tohoku.ac.jp
Fax: +81 22 795 6553
Tel: +81 22 795 6553

b Innovative Use of Light and Materials/Life, PREST, JST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

Abstract

We report on the design, synthesis and assessment of a novel biaryl-linked (BArL) molecular probe for the exploration of low-abundant target proteins for bioactive compounds based on the activity based protein profiling (ABPP) approach. Surprisingly, the performance of the BArL probe was better than that of the stepwise tagging approach that is considered to be the most effective method used in ABPP study.

Graphical abstract: “Click-made” biaryl-linker improving efficiency in protein labelling for the membrane target protein of a bioactive compound

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Article information

DOI
https://doi.org/10.1039/C0OB00843E
Article type
Communication
Submitted
06 Oct 2010
Accepted
28 Oct 2010
First published
17 Nov 2010

Org. Biomol. Chem., 2011,9, 83-85

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“Click-made” biaryl-linker improving efficiency in protein labelling for the membrane target protein of a bioactive compound

Y. Nakamura, S. Inomata, M. Ebine, Y. Manabe, I. Iwakura and M. Ueda, Org. Biomol. Chem., 2011, 9, 83 DOI: 10.1039/C0OB00843E

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