Carbon-13        labelling strategy for studying the ATP metabolism in individual yeast cells by micro-arrays for mass spectrometry

Pawel L. Urban; A. Mareike Schmidt; Stephan R. Fagerer; Andrea Amantonico; Alfredo Ibañez; Konstantins Jefimovs; Matthias Heinemann; Renato Zenobi

doi:10.1039/C1MB05248A

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Carbon-13 labelling strategy for studying the ATP metabolism in individual yeast cells by micro-arrays for mass spectrometry†

Pawel L. Urban,^ab A. Mareike Schmidt,^c Stephan R. Fagerer,^a Andrea Amantonico,^a Alfredo Ibañez,^a Konstantins Jefimovs,^d Matthias Heinemann^ce and Renato Zenobi*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry and Applied Biosciences, ETH Zurich, CH-8093 Zurich, Switzerland
E-mail: zenobi@org.chem.ethz.ch

^b Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan

^c Institute of Molecular Systems Biology, ETH Zurich, CH-8093 Zurich, Switzerland

^d Laboratory for Electronics/Metrology/Reliability, EMPA, Swiss Federal Laboratories for Material Science and Technology, CH-8600 Dübendorf, Switzerland

^e Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands

Abstract

Isotopic labelling of cellular metabolites, used in conjunction with high-density micro-arrays for mass spectrometry enables observation of ATP metabolism in single yeast cells.

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Article information

DOI: https://doi.org/10.1039/C1MB05248A
Article type: Communication
Submitted: 19 Jun 2011
Accepted: 27 Jul 2011
First published: 18 Aug 2011

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Mol. BioSyst., 2011,7, 2837-2840

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Carbon-13 labelling strategy for studying the ATP metabolism in individual yeast cells by micro-arrays for mass spectrometry

P. L. Urban, A. M. Schmidt, S. R. Fagerer, A. Amantonico, A. Ibañez, K. Jefimovs, M. Heinemann and R. Zenobi, Mol. BioSyst., 2011, 7, 2837 DOI: 10.1039/C1MB05248A

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