Jump to main content
Jump to site search

Issue 11, 2011
Previous Article Next Article

Proteomic analysis of gemcitabine-induced drug resistance in pancreatic cancer cells

Author affiliations

Abstract

Currently, the most effective agent against pancreatic cancer is gemcitabine (GEM), which inhibits tumor growth by interfering with DNA replication and blocking DNA synthesis. However, GEM-induced drug resistance in pancreatic cancer compromises the therapeutic efficacy of GEM. To investigate the molecular mechanisms associated with GEM-induced resistance, 2D-DIGE and MALDI-TOF mass spectrometry were performed to compare the proteomic alterations of a panel of differential GEM-resistant PANC-1 cells with GEM-sensitive pancreatic cells. The proteomic results demonstrated that 33 proteins were differentially expressed between GEM-sensitive and GEM-resistant pancreatic cells. Of these, 22 proteins were shown to be resistance-specific and dose-dependent in the regulation of GEM. Proteomic analysis also revealed that proteins involved in biosynthesis and detoxification are significantly over-expressed in GEM-resistant PANC-1 cells. In contrast, proteins involved in vascular transport, bimolecular decomposition, and calcium-dependent signal regulation are significantly over-expressed in GEM-sensitive PANC-1 cells. Notably, both proteinprotein interaction of the identified proteins with bioinformatic analysis and immunoblotting results showed that the GEM-induced pancreatic cell resistance might interplay with tumor suppressor protein p53. Our approach has been shown here to be useful for confidently detecting pancreatic proteins with differential resistance to GEM. Such proteins may be functionally involved in the mechanism of chemotherapy-induced resistance.

Graphical abstract: Proteomic analysis of gemcitabine-induced drug resistance in pancreatic cancer cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 30 Mar 2011, accepted on 16 Aug 2011 and first published on 06 Sep 2011


Article type: Paper
DOI: 10.1039/C1MB05125C
Mol. BioSyst., 2011,7, 3065-3074

  •   Request permissions

    Proteomic analysis of gemcitabine-induced drug resistance in pancreatic cancer cells

    Y. Chen, J. Liu, S. Lin, J. Li, S. Huang, J. Chen, J. Wu, C. Kuo, C. Wu, Y. Lu, Y. Chen, C. Fan, P. Huang, C. Law, P. Lyu, H. Chou and H. Chan, Mol. BioSyst., 2011, 7, 3065
    DOI: 10.1039/C1MB05125C

Search articles by author

Spotlight

Advertisements