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Issue 6, 2011
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Functional phosphosite screening for targeted proteinprotein interactions by combining phosphoproteomics strategies and mammalian two-hybrid assays

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Abstract

An effective new platform for phosphosite mapping and subsequent functional screening was developed to analyze the targeted proteinprotein interactions of p300 and CBP with β-catenin. Two novel functional phosphosites, Ser12 of p300 and Ser92 of CBP, were revealed to modulate p300/β-catenin and CBP/β-catenin interactions, respectively.

Graphical abstract: Functional phosphosite screening for targeted protein–protein interactions by combining phosphoproteomics strategies and mammalian two-hybrid assays

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Publication details

The article was received on 08 Feb 2011, accepted on 23 Mar 2011 and first published on 12 Apr 2011


Article type: Communication
DOI: 10.1039/C1MB05053B
Citation: Mol. BioSyst., 2011,7, 1838-1841
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    Functional phosphosite screening for targeted proteinprotein interactions by combining phosphoproteomics strategies and mammalian two-hybrid assays

    M. Guo, Z. Xia and H. Ma, Mol. BioSyst., 2011, 7, 1838
    DOI: 10.1039/C1MB05053B

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