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Issue 4, 2011
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The RNA–DNA hybrid structure determined by EPR, CD and RNase H1

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Abstract

A or B: RNA–DNA hybrids, key intermediates in gene regulation, were classified by pulsed electron–electron double resonance (PELDOR) in combination with CD spectroscopy into two classes, interpreted as A- and B-like structures. RNase H1 cleavage of these hybrids is in full agreement with these assignments, cleaving the hybrids with A-like geometry preferentially. This combined analytical approach allows the interpretation and eventually the design of more easily cleavable hybrids as needed for the antisense technology.

Graphical abstract: The RNA–DNA hybrid structure determined by EPR, CD and RNase H1

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The article was received on 04 Nov 2010, accepted on 07 Feb 2011 and first published on 19 Feb 2011


Article type: Communication
DOI: 10.1039/C0MB00258E
Citation: Mol. BioSyst., 2011,7, 1050-1052
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    The RNA–DNA hybrid structure determined by EPR, CD and RNase H1

    O. Romainczyk, B. Endeward, T. F. Prisner and J. W. Engels, Mol. BioSyst., 2011, 7, 1050
    DOI: 10.1039/C0MB00258E

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