Issue 3, 2011

The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of l-phenylalanine and its derivatives

Abstract

Using evolved pyrrolysyl-tRNA synthetase–tRNAPylCUA pairs, L-phenylalanine, p-iodo-L-phenylalanine and p-bromo-L-phenylalanine have been genetically incorporated into proteins at amber mutation sites in E. coli.

Graphical abstract: The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of l-phenylalanine and its derivatives

Supplementary files

Article information

Article type
Communication
Submitted
30 Sep 2010
Accepted
16 Dec 2010
First published
14 Jan 2011

Mol. BioSyst., 2011,7, 714-717

The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of L-phenylalanine and its derivatives

Y. Wang, W. K. Russell, Z. Wang, W. Wan, L. E. Dodd, P. Pai, D. H. Russell and W. R. Liu, Mol. BioSyst., 2011, 7, 714 DOI: 10.1039/C0MB00217H

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