Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 10, 2011
Previous Article Next Article

A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging

Author affiliations

Abstract

A portable and cost-effective real-time cardiotoxicity biosensor was developed using a CMOS imaging module extracted from a commercially available webcam. The detection system consists of a CMOS imaging module, a white LED and a pinhole. Real-time image processing was conducted by comparing reference and live frame images. To evaluate the engineered system, the effects of two different drugs, isoprenaline and doxorubicin, on the beating rate and beat-to-beat variations of ESC-derived cardiomyocytes were measured. The detection system was used to conclude that the beat-to-beat variability increased under treatment with both isoprenaline and doxorubicin. However, the beating rates increased upon the addition of isoprenaline but decreased for cultures supplemented with doxorubicin. Moreover, the response time for both the beating rates and the beat-to-beat variability of ESC-derived cardiomyocytes under treatment of isoprenaline was shorter than for doxorubicin, although the amount of isoprenaline used in the measurement was three orders of magnitude lower than that of doxorubicin. Given its ability to perform real-time cell monitoring in a simple and inexpensive manner, the proposed system may be useful for a range of cell-based biosensing applications.

Graphical abstract: A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging

Back to tab navigation

Supplementary files

Article information


Submitted
03 Feb 2011
Accepted
23 Mar 2011
First published
11 Apr 2011

Lab Chip, 2011,11, 1801-1807
Article type
Paper

A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging

S. B. Kim, H. Bae, J. M. Cha, S. J. Moon, M. R. Dokmeci, D. M. Cropek and A. Khademhosseini, Lab Chip, 2011, 11, 1801
DOI: 10.1039/C1LC20098D

Social activity

Search articles by author

Spotlight

Advertisements