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Issue 6, 2011
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Controlling the contents of microdroplets by exploiting the permeability of PDMS

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Abstract

A microfluidic device capable of exploiting the permeability of small molecules through polydimethylsiloxane (PDMS) has been fabricated in order to control the contents of microdroplets stored in storage wells. We demonstrate that protein precipitation and crystallization can be triggered by delivery of ethanol from a reservoir channel, thus controlling the protein solubility in microdroplets. Likewise quorum sensing in bacteria was triggered by delivery of the auto-inducer N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL) through the PDMS membrane of the device.

Graphical abstract: Controlling the contents of microdroplets by exploiting the permeability of PDMS

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Article information


Submitted
19 Nov 2010
Accepted
20 Jan 2011
First published
07 Feb 2011

Lab Chip, 2011,11, 1132-1137
Article type
Paper

Controlling the contents of microdroplets by exploiting the permeability of PDMS

J. Shim, S. N. Patil, J. T. Hodgkinson, S. D. Bowden, D. R. Spring, M. Welch, W. T.S. Huck, F. Hollfelder and C. Abell, Lab Chip, 2011, 11, 1132
DOI: 10.1039/C0LC00615G

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