Issue 8, 2011

A device for extraction, manipulation and stretching of DNA from single human chromosomes

Abstract

We describe the structure and operation of a micro/nanofluidic device in which individual metaphase chromosomes can be isolated and processed without being displaced during exchange of reagents. The change in chromosome morphology as a result of introducing protease into the device was observed by time-lapse imaging; pressure-driven flow was then used to shunt the chromosomal DNA package into a nanoslit. A long linear DNA strand (>1.3 Mbp) was seen to stretch out from the DNA package and along the length of the nanoslit. Delivery of DNA in its native metaphase chromosome package as well as the microfluidic environment prevented DNA from shearing and will be important for preparing ultra-long lengths of DNA for nanofluidic analysis.

Graphical abstract: A device for extraction, manipulation and stretching of DNA from single human chromosomes

Supplementary files

Article information

Article type
Communication
Submitted
15 Nov 2010
Accepted
01 Feb 2011
First published
25 Feb 2011

Lab Chip, 2011,11, 1431-1433

A device for extraction, manipulation and stretching of DNA from single human chromosomes

K. H. Rasmussen, R. Marie, J. M. Lange, W. E. Svendsen, A. Kristensen and K. U. Mir, Lab Chip, 2011, 11, 1431 DOI: 10.1039/C0LC00603C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements