Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.

Issue 6, 2011
Previous Article Next Article

Ring-shaped neuronal networks: a platform to study persistent activity

Author affiliations


Persistent activity in the brain is involved in working memory and motor planning. The ability of the brain to hold information ‘online' long after an initiating stimulus is a hallmark of brain areas such as the prefrontal cortex. Recurrent network loops such as the thalamocortical loop and reciprocal loops in the cortex are potential substrates that can support such activity. However, native brain circuitry makes it difficult to study mechanisms underlying such persistent activity. Here we propose a platform to study synaptic mechanisms of such persistent activity by constraining neuronal networks to a recurrent loop like geometry. Using a polymer stamping technique, adhesive proteins are transferred onto glass substrates in a precise ring shape. Primary rat hippocampal cultures were capable of forming ring-shaped networks containing 40–60 neurons. Calcium imaging of these networks show evoked persistent activity in an all-or-none manner. Blocking inhibition with bicuculline methaiodide (BMI) leads to an increase in the duration of persistent activity. These persistent phases were abolished by blockade of asynchronous neurotransmitter release by ethylene glycol tetraacetic acid (EGTA-AM).

Graphical abstract: Ring-shaped neuronal networks: a platform to study persistent activity

Back to tab navigation

Supplementary files

Publication details

The article was received on 27 Sep 2010, accepted on 10 Jan 2011 and first published on 04 Feb 2011

Article type: Paper
DOI: 10.1039/C0LC00450B
Citation: Lab Chip, 2011,11, 1081-1088

  •   Request permissions

    Ring-shaped neuronal networks: a platform to study persistent activity

    A. Vishwanathan, G. Bi and H. C. Zeringue, Lab Chip, 2011, 11, 1081
    DOI: 10.1039/C0LC00450B

Search articles by author