Jump to main content
Jump to site search

Issue 3, 2011
Previous Article Next Article

A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification

Author affiliations

Abstract

This paper reports a droplet microfluidic, sample-to-answer platform for the detection of disease biomarkers and infectious pathogens using crude biosamples. The platform exploited the dual functionality of silica superparamagnetic particles (SSP) for solid phase extraction of DNA and magnetic actuation. This enabled the integration of sample preparation and genetic analysis within discrete droplets, including the steps of cell lysis, DNA binding, washing, elution, amplification and detection. The microfluidic device was self contained, with all reagents stored in droplets, thereby eliminating the need for fluidic coupling to external reagent reservoirs. The device incorporated unique surface topographic features to assist droplet manipulation. Pairs of micro-elevations were created to form slits that facilitated efficient splitting of SSP from droplets. In addition, a compact sample handling stage, which integrated the magnet manipulator, the droplet microfluidic device and a Peltier thermal cycler, was built for convenient droplet manipulation and real-time detection. The feasibility of the platform was demonstrated by analysing ovarian cancer biomarker Rsf-1 and detecting Escherichia coli with real time polymerase chain reaction and real time helicase dependent amplification.

Graphical abstract: A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification

Back to tab navigation

Supplementary files

Article information


Submitted
14 Aug 2010
Accepted
07 Oct 2010
First published
03 Nov 2010

Lab Chip, 2011,11, 398-406
Article type
Paper

A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification

Y. Zhang, S. Park, K. Liu, J. Tsuan, S. Yang and T. Wang, Lab Chip, 2011, 11, 398 DOI: 10.1039/C0LC00296H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements