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Issue 21, 2011

A highly sensitive magnetite nanoparticle as a simple and rapid stem cell labelling agent for MRI tracking

Author affiliations

Abstract

A superparamagnetic iron oxide nanoparticle (SPION) was coupled to 2-aminoethyl-trimethyl ammonium (TMA) in a 2-step ligand exchange reaction to produce TMA–SPION. This particle, which has a strong positive charge, was investigated as the basis for a simple and efficient method for labelling human mesenchymal stem cells (hMSCs) for noninvasive monitoring by magnetic resonance imaging (MRI). TMA–SPION has a ζ potential of +40 mV and a hydrodynamic size of 101 nm. In addition to its long-term stability in an aqueous solution, TMA–SPION has a low cytotoxicity and favourable magnetic properties as a T2 contrast agent due to its high relaxivity. The T2 relaxivity of TMA–SPION is 4.4 times greater than the commercially available Feridex® I.V. magnetic resonance agent. Despite a short labelling time of 4 h, hMSCs are efficiently labelled with TMA–SPION without the need for a transfection agent. An in vivo MRI study of a brain infarction model confirmed the utility of TMA–SPION as an MRI tracking marker of administered hMSCs.

Graphical abstract: A highly sensitive magnetite nanoparticle as a simple and rapid stem cell labelling agent for MRI tracking

Supplementary files

Article information


Submitted
17 Jan 2011
Accepted
15 Mar 2011
First published
13 Apr 2011

J. Mater. Chem., 2011,21, 7742-7747
Article type
Paper

A highly sensitive magnetite nanoparticle as a simple and rapid stem cell labelling agent for MRI tracking

H. Kim, H. Dae, C. Park, E. O. Kim, D. Kim, I. Kim, Y. Kim and Y. Choi, J. Mater. Chem., 2011, 21, 7742 DOI: 10.1039/C1JM10247H

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