Issue 21, 2011
From the journal:

Dalton Transactions

Modification of the deoxy-myoglobin/carbonmonoxy-myoglobin UV-vis assay for reliable determination of CO-release rates from organometallic carbonyl complexes

Anthony J. Atkin,a   Jason M. Lynam,a   Benjamin E. Moulton,a   Philip Sawle,b   Roberto Motterlini,bc   Nicola M. Boyle,d   Mary T. Pryced  and  Ian J. S. Fairlamb*a  

* Corresponding authors

a Department of Chemistry, University of York, Heslington, York
E-mail: ian.fairlamb@york.ac.uk
Fax: +44 (0)1904 322516
Tel: +44 (0)1904 324091

b Vascular Biology Unit, Department of Surgical Research, Northwick Park Institute for Medical Research, Harrow, Middlesex, UK

c Department of Drug Discovery and Development, Italian Institute of Technology, Genova, Italy

d School of Chemical Sciences, Dublin City University, Dublin 9, Ireland

Abstract

The deoxy-myoglobin (deoxy-Mb)/carbonmonoxy-myoglobin (Mb-CO) UV-vis assay is the principal method used for quantifying the rates of CO release from CO-releasing molecules (CO-RMs) that might possess therapeutic benefits. Some issues emerge when the Mb-CO assay is utilized for testing CO-RMs with novel structures, which are comprehensively discussed here for the first time. Two methods for processing raw UV-vis spectroscopic data generated from the assay are presented in this paper.

Graphical abstract: Modification of the deoxy-myoglobin/carbonmonoxy-myoglobin UV-vis assay for reliable determination of CO-release rates from organometallic carbonyl complexes

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Article information

DOI
https://doi.org/10.1039/C0DT01809K
Article type
Paper
Submitted
22 Dec 2010
Accepted
24 Mar 2011
First published
27 Apr 2011

Dalton Trans., 2011,40, 5755-5761

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Modification of the deoxy-myoglobin/carbonmonoxy-myoglobin UV-vis assay for reliable determination of CO-release rates from organometallic carbonyl complexes

A. J. Atkin, J. M. Lynam, B. E. Moulton, P. Sawle, R. Motterlini, N. M. Boyle, M. T. Pryce and I. J. S. Fairlamb, Dalton Trans., 2011, 40, 5755 DOI: 10.1039/C0DT01809K

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