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Issue 5, 2011
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Studying proteinprotein interactions using peptide arrays

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Abstract

Screening of arrays and libraries of compounds is well-established as a high-throughput method for detecting and analyzing interactions in both biological and chemical systems. Arrays and libraries can be composed from various types of molecules, ranging from small organic compounds to DNA, proteins and peptides. The applications of libraries for detecting and characterizing biological interactions are wide and diverse, including for example epitope mapping, carbohydrate arrays, enzyme binding and proteinprotein interactions. Here, we will focus on the use of peptide arrays to study proteinprotein interactions. Characterization of proteinprotein interactions is crucial for understanding cell functionality. Using peptides, it is possible to map the precise binding sites in such complexes. Peptide array libraries usually contain partly overlapping peptides derived from the sequence of one protein from the complex of interest. The peptides are attached to a solid support using various techniques such as SPOT-synthesis and photolithography. Then, the array is incubated with the partner protein from the complex of interest. Finally, the detection of the protein-bound peptides is carried out by using immunodetection assays. Peptide array screening is semi-quantitative, and quantitative studies with selected peptides in solution are required to validate and complement the screening results. These studies can improve our fundamental understanding of cellular processes by characterizing amino acid patterns of proteinprotein interactions, which may even develop into prediction algorithms. The binding peptides can then serve as a basis for the design of drugs that inhibit or activate the target proteinprotein interactions. In the current review, we will introduce the recent work on this subject performed in our and in other laboratories. We will discuss the applications, advantages and disadvantages of using peptide arrays as a tool to study proteinprotein interactions.

Graphical abstract: Studying protein–protein interactions using peptide arrays

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Publication details

The article was received on 30 Jun 2010 and first published on 18 Jan 2011


Article type: Tutorial Review
DOI: 10.1039/C0CS00029A
Citation: Chem. Soc. Rev., 2011,40, 2131-2145
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    Studying proteinprotein interactions using peptide arrays

    C. Katz, L. Levy-Beladev, S. Rotem-Bamberger, T. Rito, S. G. D. Rüdiger and A. Friedler, Chem. Soc. Rev., 2011, 40, 2131
    DOI: 10.1039/C0CS00029A

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