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Issue 11, 2011
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Inkjet-printed gold nanoparticle electrochemical arrays on plastic. Application to immunodetection of a cancer biomarkerprotein

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Abstract

Electrochemical detection combined with nanostructured sensor surfaces offers potentially low-cost, high-throughput solutions for detection of clinically significant proteins. Inkjet printing offers an inexpensive non-contact fabrication method for microelectronics that is easily adapted for incorporating into protein immunosensor devices. Herein we report the first direct fabrication of inkjet-printed gold nanoparticle arrays, and apply them to electrochemical detection of the cancer biomarker interleukin-6 (IL-6) in serum. The gold nanoparticle ink was printed on a flexible, heat resistant polyimide Kapton substrate and subsequently sintered to create eight-electrode arrays costing <0.2 euro per array. The inkjet-printed working electrodes had reproducible surface areas with RSD <3%. Capture antibodies for IL-6 were linked onto the eight-electrode array, and used in sandwich immunoassays. A biotinylated secondary antibody with 16-18 horseradish peroxidase labels was used, and detection was achieved by hydroquinone-mediated amperometry. The arrays provided a clinically relevant detection limit of 20 pg mL−1 in calf serum, sensitivity of 11.4 nA pg−1 cm−2, and a linear dynamic range of 20–400 pg mL−1.

Graphical abstract: Inkjet-printed gold nanoparticle electrochemical arrays on plastic. Application to immunodetection of a cancer biomarker protein

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Article information


Submitted
08 Sep 2010
Accepted
03 Dec 2010
First published
07 Jan 2011

Phys. Chem. Chem. Phys., 2011,13, 4888-4894
Article type
Paper

Inkjet-printed gold nanoparticle electrochemical arrays on plastic. Application to immunodetection of a cancer biomarker protein

G. C. Jensen, C. E. Krause, G. A. Sotzing and J. F. Rusling, Phys. Chem. Chem. Phys., 2011, 13, 4888
DOI: 10.1039/C0CP01755H

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