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Abstract | Cited by

We report the first example of RNA labeling based on inverse electron-demand Diels–Alder reactions. Both chemically synthesized and enzymatically transcribed RNAs were successfully modified with biotin or a fluorescent label. This approach works efficiently under mild conditions in water and does not require transition metals.

Graphical abstract: Inverse electron-demand Diels–Alder reactions for the selective and efficient labeling of RNA

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