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Issue 18, 2011
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Hydroxylation of methylated CpG dinucleotides reverses stabilisation of DNA duplexes by cytosine 5-methylation

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Abstract

Cytosine-5-methylation stabilises DNA duplexes and is associated with transcriptional repression; 5-methylcytosine undergoes hydroxylation to 5-hydroxymethylcytosine, a modification of unknown biological function. Spectroscopic and calorimetric analyses show that 5-hydroxymethylcytosine introduction reverses the stabilising effect of 5-methylcytosine, suggesting that in some contexts, 5-methylcytosine hydroxylation may, along with other factors, contribute to the alleviation of transcriptional repression.

Graphical abstract: Hydroxylation of methylated CpG dinucleotides reverses stabilisation of DNA duplexes by cytosine 5-methylation

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Publication details

The article was received on 20 Dec 2010, accepted on 16 Mar 2011 and first published on 31 Mar 2011


Article type: Communication
DOI: 10.1039/C0CC05671E
Chem. Commun., 2011,47, 5325-5327

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    Hydroxylation of methylated CpG dinucleotides reverses stabilisation of DNA duplexes by cytosine 5-methylation

    A. Thalhammer, A. S. Hansen, A. H. El-Sagheer, T. Brown and C. J. Schofield, Chem. Commun., 2011, 47, 5325
    DOI: 10.1039/C0CC05671E

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