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Issue 18, 2011
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Hydroxylation of methylated CpG dinucleotides reverses stabilisation of DNA duplexes by cytosine 5-methylation

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Abstract

Cytosine-5-methylation stabilises DNA duplexes and is associated with transcriptional repression; 5-methylcytosine undergoes hydroxylation to 5-hydroxymethylcytosine, a modification of unknown biological function. Spectroscopic and calorimetric analyses show that 5-hydroxymethylcytosine introduction reverses the stabilising effect of 5-methylcytosine, suggesting that in some contexts, 5-methylcytosine hydroxylation may, along with other factors, contribute to the alleviation of transcriptional repression.

Graphical abstract: Hydroxylation of methylated CpG dinucleotides reverses stabilisation of DNA duplexes by cytosine 5-methylation

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Article information


Submitted
20 Dec 2010
Accepted
16 Mar 2011
First published
31 Mar 2011

Chem. Commun., 2011,47, 5325-5327
Article type
Communication

Hydroxylation of methylated CpG dinucleotides reverses stabilisation of DNA duplexes by cytosine 5-methylation

A. Thalhammer, A. S. Hansen, A. H. El-Sagheer, T. Brown and C. J. Schofield, Chem. Commun., 2011, 47, 5325
DOI: 10.1039/C0CC05671E

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