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Issue 20, 2011
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Standardization of Raman spectra for transfer of spectral libraries across different instruments

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Abstract

In this paper we evaluate methods for standardization of Raman spectra that are required to improve spectral correlation computations between spectra measured on different instruments. Five commercially-available 785 nm Raman spectrometers from different vendors were included in the study. These spectrometers have diverse specifications and performance levels and range in size from laboratory-based instruments to field-deployable portable and handheld platforms. Since each Raman spectrometer has different characteristics, spectra obtained on one instrument cannot readily be compared to a library acquired on a different instrument without performing various types of spectral corrections (standardization). We outline a procedure that combines previously established Raman shift and intensity correction protocols with a resolution matching step to facilitate the comparison of a centralized master library with spectra acquired on different geographically distributed Raman spectrometers. The standardization procedure is effective in reducing the inherent instrument-to-instrument variability so that spectra from different spectrometers can be compared and reliable results obtained using library-based spectral correlation methods. The findings have important implications for the ability to transfer Raman spectral libraries between instruments.

Graphical abstract: Standardization of Raman spectra for transfer of spectral libraries across different instruments

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Publication details

The article was received on 22 Jul 2011, accepted on 27 Jul 2011 and first published on 26 Aug 2011


Article type: Paper
DOI: 10.1039/C1AN15636E
Citation: Analyst, 2011,136, 4232-4240
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    Standardization of Raman spectra for transfer of spectral libraries across different instruments

    J. D. Rodriguez, B. J. Westenberger, L. F. Buhse and J. F. Kauffman, Analyst, 2011, 136, 4232
    DOI: 10.1039/C1AN15636E

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