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Issue 20, 2011
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Dynamic light scattering as an efficient tool to study glyconanoparticle–lectin interactions

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Abstract

Glyconanomaterials, an emerging class of bio-functional nanomaterials, have shown promise in detecting, imaging and targeting proteins, bacteria, and cells. In this article, we report that dynamic light scattering (DLS) can be used as an efficient tool to study glyconanoparticle (GNP)––lectin interactions. Silica and Au nanoparticles (NPs) conjugated with D-mannose (Man) and D-galactose (Gal) were treated with the lectins Concanavalin A (Con A) and Ricinus communis agglutinin (RCA120), and the hydrodynamic volumes of the resulting aggregates were measured by DLS. The results showed that the particle size grew with increasing lectin concentration. The limit of detection (LOD) was determined to be 2.9 nM for Con A with Man-conjugated and 6.6 nM for RCA120 with Gal-conjugated silica NPs (35 nm), respectively. The binding affinity was also determined by DLS and the results showed 3–4 orders of magnitude higher affinity of GNPs than the free ligands with lectins. The assay sensitivity and affinity were particle size dependent and decreased with increasing particle diameter. Because the method relies on the particle size growth, it is therefore general and can be applied to nanomaterials of different compositions.

Graphical abstract: Dynamic light scattering as an efficient tool to study glyconanoparticle–lectin interactions

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Publication details

The article was received on 08 Jun 2011, accepted on 30 Jul 2011 and first published on 22 Aug 2011


Article type: Paper
DOI: 10.1039/C1AN15469A
Analyst, 2011,136, 4174-4178

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    Dynamic light scattering as an efficient tool to study glyconanoparticle–lectin interactions

    X. Wang, O. Ramström and M. Yan, Analyst, 2011, 136, 4174
    DOI: 10.1039/C1AN15469A

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