Zinc-finger motif noncovalent interactions with double-stranded DNA characterized by negative-ion electrospray ionization mass spectrometry
Abstract
A zinc-finger motif recognizes specific sequences on the double helical structure of DNA. This sequence recognition property offers great promise for various biotechnology applications. Accordingly, it is crucially important to characterize zinc-finger binding characteristics for further developments. Although the gel shift assay or phage display is traditionally used for determining the binding characteristics of zinc-fingers for double stranded DNA, in the present study we utilize