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Issue 19, 2011
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Double isotope dilution surface-enhanced Raman scattering as a reference procedure for the quantification of biomarkers in human serum

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Abstract

Double isotope dilution surface-enhanced Raman scattering (double IDSERS) is qualified as a method for accurate and precise determination of biomarkers in human blood serum. Providing a full evaluation of the measurement uncertainty as well as traceability to a reference material sets the procedure in line with the requirements of a primary ratio method. Data evaluation is based on a partial least squares (PLS) model, whose prediction ability is validated from quantifying the uric acid concentration in both an artificial reference solution and a real human blood serum sample. With the proposed approach, the uric acid serum concentration can be determined with an uncertainty of 1.6% at a confidence level of 95%.

Graphical abstract: Double isotope dilution surface-enhanced Raman scattering as a reference procedure for the quantification of biomarkers in human serum

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Publication details

The article was received on 11 Mar 2011, accepted on 28 Jun 2011 and first published on 05 Aug 2011


Article type: Paper
DOI: 10.1039/C1AN15207F
Analyst, 2011,136, 3956-3961

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    Double isotope dilution surface-enhanced Raman scattering as a reference procedure for the quantification of biomarkers in human serum

    S. Zakel, O. Rienitz, B. Güttler and R. Stosch, Analyst, 2011, 136, 3956
    DOI: 10.1039/C1AN15207F

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