Issue 12, 2011
From the journal:

Analyst

Direct detection of RNAs in living cells using peptide-inserted Renillaluciferase

Takashi Andou,a   Tamaki Endoh,b   Masayasu Miea  and  Eiry Kobatake*a  

* Corresponding authors

a Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259, Nagatsuta, Midori-ku, Yokohama, Japan
E-mail: ekobatak@bio.titech.ac.jp
Fax: +81-45-924-5779
Tel: +81-45-924-5760

b Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojimaminamimachi, Chuo-ku, Kobe, Japan
E-mail: t-endoh@center.konan-u.ac.jp
Fax: +81-78-303-1495
Tel: +81-78-303-1314

Abstract

In this study, non-engineered RNAs were detected in living cells using bioluminescence. Two types of probe were utilized: a peptide inserted RLuc (PI-RLuc) probe and a split-RNA probe. Incorporation of the PI-RLuc and split-RNA probes enabled the direct detection of RNA introduced into living cells.

Graphical abstract: Direct detection of RNAs in living cells using peptide-inserted Renillaluciferase

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Article information

DOI
https://doi.org/10.1039/C1AN15130D
Article type
Communication
Submitted
16 Feb 2011
Accepted
18 Apr 2011
First published
03 May 2011

Analyst, 2011,136, 2446-2449

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Direct detection of RNAs in living cells using peptide-inserted Renillaluciferase

T. Andou, T. Endoh, M. Mie and E. Kobatake, Analyst, 2011, 136, 2446 DOI: 10.1039/C1AN15130D

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